Growth properties: Mixed, Adherent And Suspension. Therefore, the detection of mycoplasma cells is particularly important. Morphology: Morphology microglial. Here, we show that primarily human fibrillar alpha-synuclein increased the production and secretion of pro-inflammatory cytokines by microglial BV2 cells compared to monomeric and oligomeric alpha-synuclein. The quantification of relative band intensities from three independent experimental results was determined by densitometry. When cells (especially passage cells) are contaminated by mycoplasma, the expression of DNA, RNA and protein in the cell changes, but the growth rate and cell morphology of the cells have not changed significantly. BV-2 (mouse, C57BL/6, brain, microglial cells)ICLC catalog code: ATL03001 Continuous culture, grown as semiadherent microglial morphology SPECIES: mouse, C57BL/6 TISSUE/ORGAN: brain, microglial cells TRANSFORMED BY: recombinant retrovirus (v-raf/v-mic) DEPOSITOR: Dr E. Blasi, Dip. Subculturing: Remove and discard culture medium. 2A, left). These mycoplasma-contaminated BV2 cells exhibited a transition of cell morphology and slower proliferation, as well as increased gene expression and protein secretion of inflammatory factors. BV-2 MORPHOLOGY EXPERIMENTS. BV2 cells and primary microglia were plated at a density of 20,000 cells in 96-well (Greiner) plates. Medium: MEM +10% FBS +1% P/S. BV2 cells treated with LPS (10 μg/mL) presented a rounded amoeboid-like appearance with fine processes extending from the soma ( Fig. The ATCC Cell Biology Collection is one of the largest bioresources in the world, and offers a complex array of human, animal, insect, fish and stem cell lines from which to choose. One of the surprising and intriguing findings from our study is the differential LC3 response to LPS between microglial BV2 cells and macrophage RAW264.7 cells. BV-2 microglia were plated using serum-free Ham’s F-12 phenol red-free media on 25 mm × 75 mm glass microscope slides that had been pre-coated for 1 h with 1 μg/ml poly-D-lysine.A coated microscope slide was placed in a 10 cm Petri dish then 1 ml of cell suspension (5 × 10 5 cells/ml) was placed onto the center of the slide using a Pasteur pipette. Several studies have reported that activation of TLR4 or 7 activates autophagy in primary macrophages and the macrophage cell line ( Xu et al., 2007 ; Delgado et al., 2008 ). BV2 cells without LPS treatment presented ramified cell morphology with fine processes extending tens of microns away from the soma (Fig. Furthermore, mycoplasma-contaminated BV2 cells had decreased sensitivity to … The BV2 microglial cells were grown in Transwell inserts (pore size, 0.4 μm; Corning Life Sciences, Tewksbury, … Bar graph shows the cell viability of BV2 cells after low glucose and hypoxia compared to control ( B ). The BV2 microglial cells were co-cultured with primary cortical neurons to study the regulation of neuronal survival by the LPS-stimulated microglia. The external cell morphology appears relatively unchanged in BV2 cells exposed to low glucose and hypoxia for 4 h, 8 h and 12 h when compared with the control ( A ). 2 A, right). Biosafety: 1. These cells grow as a mixture of floating and adherent cells. BV2 cells were treated with ICSB in the presence or absence of LPS (500 ng/mL) for 20 h. Effects of ICSB on IκB phosphorylation ( A ), in LPS-stimulated BV2 cells by Western blotting. BV2 cells also preferentially phagocytosed fibrillar alpha-synuclein compared to alpha-synuclein monomers and oligomers. ATCC was entrusted with its first cell line in 1962 and has consistently attained the highest standards and used the most reliable procedures to verify every cell line since.